Effect of vincristrine sulphate on the nasal mucosa

The present work was undertaken to study the effect of vincristine sulphate on the respiratory and olfactory mucosa of the nose. Eight adult rabbits were used in this work. They were divided into two equal groups and were designated group 1 and II. The animals of group I were given a daily ingection of vincristine [0.25 mg/Kg] for five days. The animals of group II were similarly injected with the dissolving medium for vincristine and were used as controls. The animals of groups I and II were sacrificed seved days after the last injection. Decalcification of the heads was done by 5% trichloroacetic acid. The regions of the nose were excised and sections were stained with hematoxylin and eosin, PAS and Glees's silver. Administration of vincristine was followed by atropy and degeneration of olfactory epithelium, thickening of basement membrane, degeneration of mucoserous glands, thickening of blood vessels and degeneration of nerve budiles and plexuses. Possible reasons for this were put forward

Histopathological and histochemical studies of the ovary and uterus of the female dog after ligation of the supplying arteries

The present study was undertaken to study the histopathological and histochemical studies of the ovary and uterus of female dog after ligation of the supplying arteries. Twelve adult female dogs were divided into three groups. Unilateral ligation of the internal iliac, uterine and ovarian arteries was done in group I, II and III, respectively. All animals were sacrificed one month after the operation. Samples were taken from the ovaries and uterine horns of both the ligated and nonligated sides for histological and histochemical studies. Unilateral ligationof either internal iliac, uterine or ovarian arteries was followed by histopathological changes in the uterine horns of both sides. However, the degenerative changes were more marked at the horns of the ligated sides. The endometrial glands of both uterine horns showed negative PAS and alkaline phosphatase activies after unilateral ligation of the internal iliac or uterine arteries. However, after ligation of the ovarian artery, the endometrial glands of the ligated side showed weak PAS reaction and moderate alkaline phosphatase activity. Those of the contralateral side showed a strong activity. Concerning the ovary, unilateral ligationof the internal iliac or uterine arteries was followed by marked degenerative changes in the ipsilateral ovary and the contralateral ovary was normal in appearance. However, ligationof the ovarian artery was followed by atrophic changes in the ovary of the ligated side. That of the contralateral side showed compensatory hypertrophy of the graafian follicles. Ligation of the internal iliac or uterine arteries was followed by strong activity of the alkaline phosphatase enzyme in the growing follicles and corpus lutii of both ipsilateral and contralateral ovaries. There was also a strong activity of the alkaline phosphatase in the hypertrophied follicles of the contralateral ovary following ligation of the ovarian artery

Histopathological and histochemical studies of the kidney after ligation of the ureter and uretero-cutaneous anastomosis

The present work was undertaken to study the histopathological of the ureter and ureterocutancous anastomosis. Twelve adult dogs were used in this work. They were classiffied into three equal groups and were designated as group A and B and C. Unilateral ligation of the ureters for one month was done at the proximal end in group A and at the distal end in group B and C. Thereafter in group C. the ureters were transected proximal to the previous ligature and anstomosed to the skin of the anterior abdominal wall. The animals were sacrificed one month after the operation. Samples were taken for histopathological and histoche-mical studies from the kidney of the operated side and the contralateral as well. Unilateral ureteral obstruction at the distal or the proximal and induced several histopathological changes in the kidney of the operated side. However, the contralateral kidney showed compensatory hypertrophy of most malpighian corpuscles and some degenerative changes in the renal tubules. The histopathological changes were found to be more marked in the kidney following proximal ureteral obstruction. The rekease of ureteral obstruction by the process of uretero-cutaneous anastomosis was followed by regeneration of many malpighian corpuscles and some glomeruli were hypertrophied. Decrease or absence of the alkaline phosphatase activity was noted in the malipighian corpuscles and the convoluted tubules of the kidney of the ligated side. However, the contralateral kidney after ureteral obstruction and the operated kidney following uretero-cutaneous anastomosis showed moderate activity for this enzyme in the malpighian corpuscles, distal convoluted tubules and loops of Henle while a strong activity was seen in the proximal convoluted tubules. The regenerating malpighian corpuscle are functionally active as indicated by their increased enzymatic activity